Training activity information
Details
Perform a nucleic acid extraction and manual PCR, including interpretation and reporting from standard curves, troubleshooting common faults and issues
Type
Entrustable training activity (ETA)
Evidence requirements
Evidence the activity has been undertaken by the trainee repeatedly, consistently, and effectively over time, in a range of situations. This may include occasions where the trainee has not successfully achieved the outcome of the activity themselves. For example, because it was not appropriate to undertake the task in the circumstances or the trainees recognised their own limitations and sought help or advice to ensure the activity reached an appropriate conclusion.
Reflection at multiple timepoints on the trainee learning journey for this activity.
Considerations
- Quality assurance
- Internal and external quality assurance schemes
- Quantitative vs qualitative assays
- Assay limitations and uncertainty
- Common issues associated with the method, including analysis and reporting
- Root cause analysis
- Remedial and preventative actions
- Local non-conformance reporting
- Requirements for notification to MHRA, manufacturers and end users for issues identified
- Duty of candour when issues arise
Relevant learning outcomes
| # | Outcome |
|---|---|
| # 1 |
Outcome
Select and perform tests to investigate common infections from a range of sample types following guidance and fulfilling health and safety requirements |
| # 4 |
Outcome
Perform and interpret molecular techniques for the investigation of common infections following guidance and fulfilling health and safety requirements |
| # 6 |
Outcome
Evaluate assay performance according to quality assurance frameworks |