Competency information
Details
Perform laboratory methods for HLA antibody definition, including:
- Complement dependent cytotoxicity
- ELISA
- Flow cytometry
- xMAP Technologies(Luminex)
Considerations
- Operational and performance characteristics of each assay technique.
- Strengths and weaknesses of each assay technique.
- Clinical significance of HLA and non- HLA antibodies in transplantation:
- Class I and Class II
- IgM allo/autoantibodies
- red cell antibodies
- endothelial cell antibodies
- How to assure the quality of personal practice.
Relevant learning outcomes
| # | Outcome |
|---|---|
| # 1 | Outcome Select and perform laboratory tests for Human Leukocyte Antigen (HLA) antibody identification, definition and data analysis. |
| # 2 | Outcome Select and perform laboratory tests required for the clinical work up of patients awaiting solid organ transplantation, including cross matching for solid organ transplantation. |
| # 3 | Outcome Perform the clinical and laboratory work up of patients referred for investigation of platelet refractoriness. |
| # 5 | Outcome Apply specific accreditation standards governing the use of serological practices. |