Competency information

Details

Prepare consumables and media for oocyte collection; fertilisation checks; embryo culture; embryo transfer; embryo cryopreservation and thawing/warming.

Considerations

  • An in-depth knowledge of water purity, osmolality, pH, temperature, protein supplements, antibiotics and microbiological contamination and their effects on oocyte and embryo viability in culture.
  • A comprehensive knowledge of the physiological requirements of an embryo at the different stages of development in culture.

Relevant learning outcomes

# Outcome
# 1 Outcome Use sterile technique to prepare culture dishes appropriate for gametes and embryos.
# 2 Outcome Culture embryos to maintain viability.