Competency information
Details
Perform and interpret swim-up techniques and density gradient separation of sperm samples of different quality, including:
- antisperm antibody-positive samples
- retrograde samples;
- severe oligoasthenozoospermic samples (for cryopreservation);
- frozen samples, including donor samples.
Considerations
- The importance of how suboptimal samples may affect future outcome.
- The different methods that can be applied to prepare semen samples of differing qualities.
- The cost effectiveness and yields associated with different techniques.
- How cryoprotectants may affect the quality of sperm and how osmotic shock may occur during the process.
- Implications of incorrect use of cryoprotectants on sample quality and outcomes.
Relevant learning outcomes
| # | Outcome |
|---|---|
| # 1 | Outcome Review a set of case notes and recommend a patient’s pathway, including treatment options based upon clinical presentation. |
| # 3 | Outcome Handle gametes correctly to maintain viability and perform different methods of sperm preparation techniques within the statutory and regulatory framework. |
| # 4 | Outcome Perform quality control checks within the laboratory and the cryofacility. |