Competency information

Details

Perform sperm cryopreservation Correctly use and handle liquid nitrogen and associated cryogenic equipment.

Considerations

  • The required consents that need to be completed before any samples can be stored and the implications for the centre if consents are not completed.
  • The principles of cryobiology, including the physical and chemical processes occurring during cryopreservation and thawing, and the use and properties of cryoprotectants.
  • Were and why sperm losses are most likely to occur during the cryopreservation process.
  • The health and safety regulations to be applied when handling liquid nitrogen.
  • The safe working practices in a cryofacility.
  • The regulatory requirements with regard to screening and storage limits.

Relevant learning outcomes

# Outcome
# 2 Outcome Perform sperm preparation, cryopreservation and thawing, post thaw preparation within the statutory and regulatory framework.
# 3 Outcome Handle gametes correctly to maintain viability and perform different methods of sperm preparation techniques within the statutory and regulatory framework.
# 4 Outcome Perform quality control checks within the laboratory and the cryofacility.