Competency information

Details

Thaw semen samples and compare pre-freeze and post-thaw parameters.

Considerations

  • How cryoprotectants may affect the quality of sperm and how osmotic shock may occur during the process.
  • Implications of incorrect use of cryoprotectants on sample quality and  outcomes.
  • The physiological changes that occur in sperm during cryopreservation.
  • Assessment of post-thaw samples and whether these are suitable for use in sperm donation.
  • How removal of cryoprotectants may affect the quality of sperm and how osmotic shock may occur during the process.

Relevant learning outcomes

# Outcome
# 2 Outcome Perform sperm preparation, cryopreservation and thawing, post thaw preparation within the statutory and regulatory framework.
# 3 Outcome Handle gametes correctly to maintain viability and perform different methods of sperm preparation techniques within the statutory and regulatory framework.
# 4 Outcome Perform quality control checks within the laboratory and the cryofacility.
# 5 Outcome Analyse key performance indicators with respect to defined outcomes then identify, troubleshoot and solve any problems.