Competency information
Details
Analyse and interpret appropriate assays for the detection of gene fusions.
Considerations
- Health and safety requirements for processing of samples
- Preparation of slides for analysis from fresh material and paraffin embedded blocks Selection of the appropriate area for analysis (eg morphological assessment of H&E stained slide)
- Principles of technologies used for identification of gene fusions including g-band analysis, FISH, RT-PCR and RNA-Seq
- Best practice guidelines for interpretation of gene fusion analysis techniques (eg breakapart and dual fusion FISH probes)
- Health and safety requirements for use of appropriate equipment (eg fluorescence microscope)
- Factors affecting quality of samples for analysis Internal quality control for assays
- Importance of external quality assurance
- Role of validation and verification of procedures and equipment
- Understanding of assay accreditation and ISO 15189 or equivalent
- National guidelines for pathways and procedures
Relevant learning outcomes
# | Outcome |
---|---|
# 3 | Outcome Apply the appropriate testing strategy that would lead to the diagnosis of a range haematological malignancies |
# 4 | Outcome Implement the use of appropriate assays for the diagnoses and monitoring of a range of haematological malignancies |