Haemopoietic Stem Cell Collection, Processing, Storage and Use

Details

Title

Haemopoietic Stem Cell Collection, Processing, Storage and Use

Type

Stage Two

Module code

HLS196

Requirement

Compulsory

Module objective

By the end of the training period with respect to haemopoietic stem cell collection, processing, storage and use, the trainee will be able to:

• analyse, synthesise, critically evaluate and apply knowledge
• perform, adapt and master a range of technical and clinical skills and procedures
• demonstrate the attitudes and behaviours necessary for professional practice as a consultant clinical scientist dealing with the complexities, uncertainties and tensions of professional practice at this level

Knowledge and understanding

By the end of the training period the trainee will be able to demonstrate the ability to analyse, critically evaluate and synthesise relevant knowledge and its application to their professional practice in relation to:

• the application of autologous and allogeneic (related and unrelated) stem cell transplantation for the treatment of malignant and non-malignant disorders
• immunological and haematological reconstitution post-transplant
• international standards for cellular therapy, product collection, processing and administration
• ethical and regulatory aspects of stem cell transplantation including appropriate consent and the role of the Human Tissue Act
• selection criteria for related and unrelated adult donors and cord blood including the role of HLA matching and cell dose
• the collection and transport bone marrow and mobilised peripheral blood stem cells
• principles of cell purification, enrichment, depletion and cryopreservation
• use of flow cytometry to measure stem cell dose and viability
• the main causes of post-transplant mortality and morbidity including:
  • relapse
  • infection
  • graft versus host disease
• immunological consequences of allogeneic stem cell transplantation including:
  • graft versus leukaemia effect
  • graft versus host disease
• graft rejection
• testing, manipulation and cryogenic storage of stem cell donations including the measures taken to avoid product contamination

Technical and clinical skills

By the end of the training period the trainee will be able to demonstrate a critical understanding of current relevant research, theory and knowledge and its application to the performance of a range of technical procedures and laboratory skills and will be able to:

• adapt and master the collection, processing, storage and use of haemopoietic stem cells
• select and provide blood components as required for stem cell processing
• manage the safe handling of stem cell donations and products, including the use of dry shippers

By the end of the training period trainees will, in respect of clinical and laboratory transfusion, be able to perform a range of clinical skills and will be able to:

• advise on the clinical significance of red cells in ABO incompatible stem cell transplants