Molecular Pathology for Histopathology 2

Details

Title

Molecular Pathology for Histopathology 2

Type

Stage Two

Module code

HLS303

Requirement

Compulsory

Module objective

By the end of the training period trainees will, in respect to background and principles, be able to:

• Analyse, synthesise, evaluate and apply knowledge
• Perform a range of technical and clinical skills and procedures
• Demonstrate the attitudes and behaviours necessary for professional practice as a consultant clinical scientist dealing with the complexities, uncertainties and tensions of professional practice at this level.

Knowledge and understanding

General

• Describes the origins and consequences of germline variation and somatic mutations, including DNA methylation and gene expression changes

Fundamentals of databases and bioinformatics

• Demonstrates ability to recall the basic molecular databases

Use of histology samples

• Describes how histological samples are taken and prepared, and how nucleic acids are extracted from them

Technology

• Outlines the principles and limitations of the most up-to-date molecular methods

Molecular Tests

• Describes molecular tests currently performed on histological samples, including the limitations of these tests and of tests that are anticipated in the near future

Technical and clinical skills

General

• Demonstrates the origins of and justifications for molecular tests

Fundamentals of databases and bioinformatics

• Summarises the use of data and identify relevant data from public sources

Use of histology samples

• Demonstrates practical understanding of undertaking the appropriate sample collection, retrieval and preparation for the common molecular tests, whether performed on extracted nucleic acid or in situ

Technology

• Demonstrates practical knowledge of sequencing, PCR, microarrays (DNA and RNA), in situ hybridisation, mutation detection

Molecular Tests

• Demonstrates the demand for molecular tests and the modes of supply
• Describes and explains common molecular tests including some of the common pitfalls and how to avoid them
• Illustrates the significance of common molecular tests