Fundamentals of Molecular Biology

Details

Title

Fundamentals of Molecular Biology

Type

Stage One

Module code

HLS376

Requirement

Compulsory

Module objective

By the end of the training period trainees will, in respect to background and principles, be able to:

• Analyse, synthesise, evaluate and apply knowledge
• Perform a range of technical and clinical skills and procedures
• Demonstrate the attitudes and behaviours necessary for professional practice as a consultant clinical scientist dealing with the complexities, uncertainties and tensions of professional practice at this level.

Knowledge and understanding

• Understands principles of common molecular pathology techniques
• Understands principles of electron microscopy
• Demonstrates understanding of the origins and consequences of germ-line variation and somatic mutations, including DNA methylation and gene expression changes
• Demonstrates knowledge of basic molecular databases
• Demonstrates knowledge of how histological samples are taken, prepared and of how nucleic acids are extracted from them
• Understands the principles of the most up-to-date molecular methods
• Demonstrates knowledge of molecular tests currently performed on histological samples, including the limitations of these tests and of tests that are anticipated in the near future

Technical and clinical skills

• Demonstrates ability to understand origins of, and justifications for, molecular tests
• Demonstrates ability to retrieve relevant data from public sources
• Demonstrates ability to undertake the appropriate sample collection, retrieval, and preparation for the common molecular tests, whether performed on extracted nucleic acid or in situ
• Demonstrates knowledge of sequencing, PCR, microarrays (DNA and RNA), in situ hybridisation and mutation detection
• Demonstrates ability to assess the demand for molecular tests and the modes of supply