Competency information
Details
Use local molecular based techniques in accordance with quality standards for intermediate resolution HLA typing.
Considerations
- How to assure the quality of personal practice.
- Clinical indications for which HLA typing is required.
- The local repertoire, source of requests and turnaround times for investigations performed by the training H&I laboratory.
- Internal quality controls and external quality assessment measures appropriate to the methods used.
- Correct and safe use of equipment used.
- Principles of PCR.
- Principles of HLA typing assays, for example:
- Sequence specific primers (SSP)
- DNA probe based hybridisation
- Real time PCR
- Sequence based typing (SBT)
- Next generation sequencing
Relevant learning outcomes
| # | Outcome |
|---|---|
| # 1 | Outcome Select and perform HLA typing methods and HLA loci to be tested for solid organ and haematopoietic stem cell transplantation, associated diseases and pharmacogenetic reactions according to current practice. |
| # 2 | Outcome Prepare good quality DNA template for HLA typing techniques. |
| # 3 | Outcome Identify the correct specimen tubes for HLA typing and be able to troubleshoot issues around DNA extraction. |
| # 4 | Outcome Perform all aspects of the current range of molecular techniques available for immunogenetic testing. |