Competency information
Details
Analyse and interpret assays for the detection of gene fusions in lymphoma samples
Considerations
- Health and safety requirements for processing of paraffin embedded samples
- Preparation of slides for analysis from paraffin embedded blocks
- Selection of the appropriate area for analysis (eg morphological assessment of H&E stained slide)
- Principles of technologies used for identification of gene fusion in FFPE derived lymphoma
- High grade B cell lymphoma with MYC and BCL2 and/or BCL6 rearrangement
- Best practice guidelines for interpretation of gene fusion analysis techniques (eg breakapart and dual fusion FISH probes)
- Health and safety requirements for use of appropriate equipment (eg fluorescence microscope)
- Factors affecting quality of samples for FISH Internal quality control for assays Importance of external quality assurance
- Role of validation and verification of procedures and equipment
- Understanding of assay accreditation and ISO 15189 or equivalent
- National guidelines for pathways and procedures
Relevant learning outcomes
| # | Outcome |
|---|---|
| # 3 | Outcome Apply the appropriate testing strategy that would lead to the diagnosis of a range haematological malignancies |
| # 4 | Outcome Implement the use of appropriate assays for the diagnoses and monitoring of a range of haematological malignancies |