Competency information
Details
Perform molecular investigations for identifying fusion gene translocations which may impact patient management.
Considerations
- Health and safety requirements for processing cancer samples.
- Scientific principles of immunohistochemistry and FISH.
- Molecular and cellular changes in cancer detected by immunohistochemistry and FISH.
- Preparation of slides for immunohistochemistry and FISH.
- Use of microscopes.
- Limitations of each procedure including limit of detection, sensitivity and specificity.
- Factors affecting the quality of samples for immunohistochemistry and FISH.
- The use of sequence based strategies for the detection of gene fusions
- Interpretation of results.
- Integration of results with other laboratory test results.
- Therapeutics available and impact of results Internal quality controls for assays.
- Importance of external quality assurance schemes.
- Role of validation and verification of procedures and equipment.
- Laboratory accreditation schemes – ISO 15189 or equivalent.
- National guidelines for care pathways and procedures.
Relevant learning outcomes
| # | Outcome |
|---|---|
| # 4 | Outcome Compare the routes of referral of patients for genomic testing from a range of clinical settings. |
| # 6 | Outcome Appreciate the role of ctDNA in diagnosis and monitoring of disease |